Evaluation of Antibiotic Efficacy Using Electron Microscopy: Morphological Effects of Guanylureido Cephalosporin, Chlorobenzoylureido Cephalosporin, BL-P1654, and Carbenicillin on Pseudomonas aeruginosa

摘要

The response of Pseudomonas aeruginosa to carbenicillin, BL-P1654, and two cephalosporins (112384 and 112883) was evaluated by minimal inhibitory concentration determinations, [14C]leucine uptake studies, morphological studies of colonial growth, and mouse intraperitoneal inoculations. Spheroplast formation and bacterial lysis were not the early response; instead, cell division was inhibited and long filaments were formed. Spheroplast formation and bacterial lysis were not observed in the first 7 h of incubation in minimal inhibitory concentrations of antibiotic.